Adrienne Bartholomew, 35, and James Brothers, 33, both of Vermilion Township, appeared in court Thursday afternoon. They're charged with two counts each of involuntary manslaughter, five counts child endangering and five counts patient endangerment in Erie County Common Pleas Court.
The child's grandmother, Deborah Nelson, 62, Vermilion Township, also pleaded not guilty to her charges, which included an additional count of involuntary manslaughter.
Isaac Brothers-Bartholomew died of malnourishment at the family's Risden Road home on Nov. 6.
He weighed only 12 pounds, about half the weight of a typical 18-month-old child.
Nelson was allegedly the child's healthcare provided. She also cared for two more of the couple's seven total children.
Five more of Isaac's sibling were were temporarily placed in their grandmother's care the day after Isaac's death, but when deputies and social workers went to check on the children they were wearing feces- and urine-soaked diapers and their beds were soaked, too.
Then they were taken to Rainbow Babies and Children's Hospital in Cleveland where they were also treated for malnutrition.
Two of them remain in the hospital. Isaac's other four siblings have been placed in foster care.
Brothers and Bartholomew are being held on $280,000 bonds. Nelson is being held on a $320,000 bond.
For more on Thursday's hearing and future court proceedings, pick up Friday's copy of the Register.